A fluorometric assay to estimate pea leaf weevil (Coleoptera: Curculionidae) larval feeding damage using leghaemoglobin in root nodules of faba bean (Fabaceae)
نویسندگان
چکیده
Abstract The pea leaf weevil, Sitona lineatus Linnaeus (Coleoptera: Curculionidae), is an invasive pest of field pea, Pisum sativum Linnaeus, and faba bean, Vicia (Fabaceae). Larvae feed on leguminous root nodules associated Rhizobium leguminosarum Frank (Rhizobiacea) nitrogen-fixing bacteria. Larval feeding causes economic damage, but the current method to assess nodule damage laborious. Leghaemoglobin, oxygen-carrying molecule in nodules, degraded as larvae feed. Measurement leghaemoglobin could be alternative estimate larval damage. Here, we developed a fluorometric assay measure variation haem fluorescence, which relates content, from nodulated roots. Roots were collected caged bean plants with or without weevil infestation. Faba yield fluorescence inversely correlated percent damaged nodules. A plant growth score was positively fluorescence. This can used pulse crops potentially other biotic stresses, it may have wider application studies nitrogen fixation.
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ژورنال
عنوان ژورنال: Canadian Entomologist
سال: 2022
ISSN: ['1918-3240', '0008-347X']
DOI: https://doi.org/10.4039/tce.2022.31